Efficient method for regenerating cotton from cultured cells

Details Efficient method for regenerating cotton from cultured cells Efficient method for regenerating cotton from cultured cells

A - Human Necessities

01

H

47/4, 195/47

A01H 1/02 (2006.01) A01G 7/00 (2006.01) A01H 4/00 (2006.01)

Patent

CA 1309367

5-16709/=/CGC 1314 An Efficient Method for Regenerating Cotton From Cultured Cells Abstract Method for producing pro-embryonic cotton cell masses in liquid sus- pension comprising the steps of: (a) inducing cotton callus formation by placing cotton plant tissue on a suitable callus induction medium at about 20° to 40°C with sufficient subcultures to prevent browning; (b) suspending up to 40 mg/ml of the callus in a suitable liquid pro- embryonic cell mass inducing medium comprising a relatively low concen- tration of at least one auxin until clumpy aggregates of pro-embryonic cell masses form and begin rapidly to proliferate; and (c) transfering the rapidly proliferating clumpy aggregates of pro- embryonic cell masses to a liquid nutrient medium capable of producing smaller, more finely dispersed pro-embryonic cell masses, said nutrient medium comprising a relatively high concentration of at least one auxin. The pro-embryonic cell masses are capable of regeneration into mature embryos, plantlets and whole plants.

583202

Affiliated with

Also associated with

Rating

Efficient method for regenerating cotton from cultured cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Efficient method for regenerating cotton from cultured cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Efficient method for regenerating cotton from cultured cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1177516