A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
A01H 4/00 (2006.01)
Patent
CA 2383944
The in vitro plant regeneration frequencies for immature scutella, leaf- bases/apical meristems (LB/AM) and mature embryos of four commercially important barley genotypes were compared. Production of shoots from mature embryos or calli of LB/AM incubated on media containing 1.0 or 2.0 mg/L 6-benzyiaminopurine (BAP) were comparable to regeneration frequencies obtained for scutella-derived calli of the same genotypes. Incubation of excised mature embryos and LB/AM on media containing an alternative plant growth regulator, thidiazuron (TDZ), resulted in an increased shoot production. However, TDZ treatment did not stimulate plant regeneration from calli derived from scutella or LB/AM. Shoots formed from TDZ- treated mature embryos and LB/AM were induced without a callus interphase and the in vitro culture system gave a three- to sight-fold higher regeneration frequency than recorded for scutella-derived calli on BAP medium. The simplicity and rapid development of shoots using the mature embryo system strongly favours its use over commonly used regeneration systems.
Baga Monica
Chibbar Ravindra
Fowler D. Brian
Fox Stephen L.
Ganeshan Seedhabadee
Anderson J. Wayne
Fowler D. Brian
Fox Stephen L.
Harvey Bryan L.
Hucl Pierre J.
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