Efficient prokaryotic expression system

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.22, 195/1.

C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01)

Patent

CA 1338366

A novel highly effective prokaryotic expression system is exemplified specifically by being used to produce the useful enzyme .beta.-glucuronidase (BG). This system uses a hybrid plasmid comprising BG gene promoter DNA. The level of expression of BG by an E. coli K-12 derivative host is in the 50% of total cellular protein range. The invention expression system also can be used to express other useful proteins, as disclosed herein.

502062

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