Efficient protein expression system

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) A61K 39/09 (2006.01) C07K 14/315 (2006.01) C12N 15/31 (2006.01) C12P 21/02 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2463008

Nucleic acid expression control sequence cassettes and vectors containing the same are provided for use in making abundant quantities of recombinant polypeptides of interest. The modified transcriptional control sequences, which include a T5 promoter sequence, are highly stable and can be used in a variety of vectors, such as plasmids.

La présente invention concerne des cassettes de séquence de commande d'expression d'acide nucléique et des vecteurs les contenant, utilisés pour produire de grandes quantités de polypeptides de recombinaison présentant un intérêt. Les séquences de commande transcriptionnelles modifiées, qui comprennent une séquence de promoteur T5, sont très stables et peuvent être utilisées dans une variété de vecteurs, tels que des plasmides.

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