Efficient riboflavin production with yeast

C - Chemistry – Metallurgy – 12 – P

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195/118, 195/34.

C12P 25/00 (2006.01) C12N 1/16 (2006.01) C12N 15/04 (2006.01)

Patent

CA 1305679

16 EFFICIENT RIBOFLAVIN PRODUCTION WITH YEAST ABSTRACT OF THE DISCLOSURE A method of systematically generating and isolating highly flavinogenic strains of Candida flareri. The strains Candida flareri GA18Y8-6#2 dgr and Candida flareri GA18Y8-6#2#11 have been developed and produce yields of ca. 7.0 to 7.5 grams per liter per 6 days. The method includes a combination of iterative mutage- nizing steps and protoplast fusion steps performed on the parent strain and the descendant strains which are selected following each step according to a screening protocol. AAAAA1/00950-2

525911

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