Electro-insertion of proteins into animal cell membranes

Details Electro-insertion of proteins into animal cell membranes Electro-insertion of proteins into animal cell membranes

C - Chemistry, Metallurgy

12

N

167/104, 195/34.

C12N 5/00 (2006.01) A61K 9/50 (2006.01) A61K 35/12 (2006.01) C12N 5/08 (2006.01) C12N 13/00 (2006.01)

Patent

CA 1327174

ABSTRACT OF THE DISCLOSURE The present invention concerns a method of incorporating a protein into a cell membrane comprising exposing a cell to an electric field while the cell is suspended in an electro-insertion medium in the presence of a buffered solution (suspension) of the protein(s) to be inserted, the protein bearing a hydrophobic membrane spanning sequence and then contacting the resultant cell with a resealing medium.

614874

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