Electrocatalytic nucleic acid hybridization detection

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2616259

The detection of specific nucleic acid sequences using electrochemical readout would permit the rapid and inexpensive detection and identification of bacterial pathogens and the analysis of human genes. A new assay developed for this purpose is described that harnesses an electrocatalytic process to monitor nucleic acid hybridization. Furthermore, the new assay when used on nanoscale electrodes, provides ultrasensitive detection of nucleic acids.

L'invention concerne la détection de séquences d'acides nucléiques spécifiques au moyen d'un affichage électrochimique permettant de détecter et d'identifier rapidement et de manière bon marché des pathogènes bactériens et d'analyser des gènes humains. L'invention concerne également un dosage développé à ces fins, lequel met en oeuvre un procédé électrocatalytique aux fins de surveillance de l'hybridation des acides nucléiques. De plus, le dosage permet d'obtenir, quand il est utilisé sur des électrodes nanométriques, une détection ultrasensible des acides nucléiques.

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