C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/37 (2006.01) G01N 27/327 (2006.01)
Patent
CA 2702969
Compositions and methods for the detection of enzymes using the nuclear reorganization energy, lambda, of an electron transfer process. The method comprises adding a test sample comprising a target enzyme to a solid support comprising an electrode, the electrode comprising a self-assembled monolayer (SAM), a covalently attached eletroactive active moiety (EAM) comprising a transi metal complex with an EO, and a plurality of substrates attached to said electrode, wherein said substrates are substrates of said enzyme. The method further comprises contacting said target enzyme and said substrates to form a plurality of reactants and determining the presence of said enzyme by measuring a change of said EO.
L'invention concerne des compositions et procédés pour la détection d'enzymes utilisant l'énergie de reconnaissance nucléaire, lambda, d'un processus de transfert d'électrons. Le procédé comprend l'addition d'un échantillon d'essai renfermant une enzyme cible à un support solide renfermant une électrode, l'électrode renfermant une monocouche auto-assemblée (SAM), une fraction active électroactive (EAM) attachée de manière covalente renfermant un complexe de métal de transition avec un OE, et une pluralité de substrats attachée à ladite électrode, lesdits substrats étant des substrats de ladite enzyme. Le procédé comprend en outre la mise en contact de ladite enzyme cible et desdits substrats pour former une pluralité de réactifs et déterminer la présence de ladite enzyme par mesure d'une modification dudit OE.
Ahrens Michael J.
Bertin Paul A.
Georganopoulou Dimitra
Gray Harry B.
Meade Thomas J.
Ohmx Corporation
Smart & Biggar
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