Electrochemical probes for detection of molecular...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 7/08 (2006.01) C07K 17/00 (2006.01) C12Q 1/00 (2006.01) G01N 27/26 (2006.01) G01N 27/327 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2279571

This invention relates to methods and apparatus for performing electrochemical analyses. The invention provides an electrochemical apparatus for performing potentiometric analyses for detecting specific binding between a first member of a biological binding pair immobilized on an electrode and a second member of a biological binding pair that is electrochemically labeled, in the presence of an electrochemical mediator. Methods for using the apparatus of the invention for performing binding and competition binding assays are provided. The invention also provides methods for performing high throughput screening assays for detecting inhibition of specific binding between the members of the biological binding pair for use in drug development, biochemical analysis and protein purification assays.

L'invention concerne des procédés et des appareils servant à effectuer des analyses électrochimiques. L'invention porte sur un appareil électrochimique permettant d'effectuer des analyses potentiométriques afin de détecter une liaison spécifique entre un premier élément d'une paire de liaisons biologiques immobilisée sur une électrode et un second élément d'une paire de liaisons biologiques électrochimiquement marquée, en présence d'un médiateur électrochimique. Font aussi l'objet de cette invention des procédés utilisant l'appareil pour effectuer des analyses de liaisons et de liaisons compétitives. L'invention porte également sur des procédés servant à effectuer des analyses de dépistage à haut rendement, permettant de détecter une inhibition de liaison spécifique entre les éléments de la paire de liaisons biologiques, et utiles pour la mise au point de médicaments, l'analyse biochimique et les analyses de purification de protéines.

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