Electrochemical treatment of nucleic acid containing solutions

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2128686

A process is described for denaturing native double-stranded nucleic acid material into its individual anode in an electrochemical cell. The process is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process employs a promoter which is an inorganic multivalent cation such as magnesium ions to speed denaturation. The process may be used in the detection of nucleic acid by hybridrising with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.

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