Electrofusion of cells and apparatus therefore

Details Electrofusion of cells and apparatus therefore Electrofusion of cells and apparatus therefore

C - Chemistry, Metallurgy

12

N

C12N 15/02 (2006.01) B01L 3/02 (2006.01) C12M 1/26 (2006.01) C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 5/12 (2006.01) C12N 5/16 (2006.01) C12N 13/00 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) A61K 35/12 (2006.01) B03C 5/00 (2006.01)

Patent

CA 2484629

The present invention relates to a method and apparatus for fusing first and second cells. In particular, the method includes positioning the first and second cells between two electrodes (35) in a fluid filled container (40) using a pipette system (33), with the first and second cells being held separated from each electrode. Once this has been achieved a current having a predetermined waveform is applied to the electrodes (35) to generate a predetermined electric field thereby causing the cells to fuse.

L'invention porte sur un procédé et un appareil de fusion d'une première cellule et d'une deuxième cellule. Ledit procédé consiste en particulier à placer les deux cellules entre deux électrodes (35) dans un récipient (40) rempli d'un liquide à l'aide d'un système (33) de pipettes, les deux cellules étant maintenues séparées des électrodes. Cela étant, on applique aux électrodes (35) un courant de forme d'onde prédéterminée créant un champ électrique prédéterminé qui entraîne la fusion des cellules.

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