Electrolyte solutions and in vitro use thereof

C - Chemistry – Metallurgy – 12 – N

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167/322, 195/40,

C12N 9/00 (2006.01) A61K 33/10 (2006.01) A61K 33/14 (2006.01) C12N 5/00 (2006.01)

Patent

CA 1280082

ABSTRACT Processes and compositions are provided for in vitro use in tissue culture media (preferred), perfusion media, and incubation media. Thus, balanced salt solutions are provided wherein the milliequivalent ratio of sodium cations to chloride anions is normalized and wherein optionally at least one near-equilibrium couple is incorporated (bicarbonate-/carbon dioxide, L-lactate-/pyruvate-, and d-betahydroxybutyrate-/acetoacetate-. Regulation of intracellular and extracellular pH, and normalization of intracellular redox state and phosphorylation state is achievable.

484983

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