Electron-deficient nitrogen heterocycle-substituted...

Details Electron-deficient nitrogen heterocycle-substituted... Electron-deficient nitrogen heterocycle-substituted...

C - Chemistry, Metallurgy

07

D

C07D 405/14 (2006.01) C07H 19/06 (2006.01) C07H 19/16 (2006.01) C07H 21/00 (2006.01) C09B 11/28 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2368495

The invention provides compositions electron-deficient nitrogen heterocycle- substituted fluorescein dyes and meth-ods in which the dyes are conjugated to substrates and used as detection labels in molecular biology experiments. The electron-de-ficient nitrogen heterocycles include pyridine, quinoline, pyrazine, and the like. Substrates include polynucleotides, nucleosides, nucleotides, peptides, proteins, carbohydrates, and ligands.

L'invention concerne des compositions à base de fluorescéïne substituée par un hétérocycle azote pauvre en électrons, et des procédés dans lesquels lesdits colorants sont conjugués à des substrats et utilisés comme étiquettes de détection dans des expériences de biologie moléculaire. Les hétérocycles azote pauvre en électrons comprennent pyridine, quinoline, pyrazine, et analogue. Les substrats comprennent des polynucléotides, des nucléosides, des nucléotides, des peptides, des protéines, des carbohydrates, et des ligands.

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