Electron transfer dissociation for biopolymer sequence analysis

H - Electricity – 01 – J

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Details

H01J 49/04 (2006.01) G01N 27/00 (2006.01) G01N 33/483 (2006.01) G01N 33/68 (2006.01) H01J 49/42 (2006.01)

Patent

CA 2559260

The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.

La présente invention concerne un nouveau procédé destiné à fragmenter des ions dans un spectromètre de masse au moyen d'une dissociation par transfert d'électrons, et à réaliser une analyse de séquence de peptides et de protéines par spectrométrie de masse. Dans le cas de peptides, l'invention favorise la fragmentation le long du squelette peptidique et permet de déduire la séquence d'acides aminés de l'échantillon, y compris les résidus d'acides aminés modifiés, au moyen d'un dispositif à champ RF.

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