Electrophoresis cell for multiple slab gels

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2396970

Electrophoresis is performed simultaneously in a multitude of slab gels in a single cell that supports the slab gels vertically and parallel to each other while immersed in a buffer solution, and that applies a voltage to all gels simultaneously through a single pair of plate electrodes. Temperature control is achieved by circulating the buffer solution upward through the cell, entering near the base of each slab gel and leaving near the top, and cooling the circulating buffer solution with a tube heat exchanger positioned on the floor of the cell.

On procède simultanément, dans le cadre de cette invention, à une électrophorèse sur plusieurs plaques de gel et ce, dans une seule unité renfermant des plaques de gel immergées dans une solution tampon, disposées verticalement et parallèlement les unes aux autres. On fait passer en même temps sur toutes les plaques un courant d'une certaine une tension par le moyen de deux électrodes à plaque. On régule la température en faisant circuler la solution tampon vers le haut, celle-ci pénétrant par le fond et étant évacuée par le dessus de l'unité. Un tube échangeur de chaleur, placé au fond de l'unité, permet de refroidir cette solution tampon en circulation.

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