Electrophoresis gels and gel holders having fiber spacers...

G - Physics – 01 – N

Patent

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Details

G01N 27/453 (2006.01) G01N 21/64 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2239988

Gel holders for electrophoresis gels are made using clad fibers, particularly glass fibers as spacers between substrates. A plurality of fibers with a high- melting interior core and a low-melting external cladding are placed between a first planar substrate and a second planar substrate. The fibers are heated to a temperatrure sufficient to at least soften the exterior cladding of the fibers without softening the interior core of the fibers, and then cooled while they are in contact with the first and second substrates to resolidify the exterior cladding. This adheres the fibers to the first and second substrates, and forms a gel chamber between said first and second substrates. The gel chamber has a thickness defined by interior core of the fibers. The fibers may be heated before or after the second substrate is placed over the top of the fibers. The gel holders thus formed may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

Des supports pour gels pour électrophorèse font appel à des fibres enrobées, en particulier des fibres de verre, servant d'éléments d'espacement entre les substrats. Plusieurs fibres avec un noyau interne à point de fusion élevé et un enrobage externe à point de fusion bas sont placées entre des premier et second substrats plats. Les fibres sont chauffées à une température suffisante pour ramollir au moins l'enrobage externe des fibres sans ramollir le noyau interne des fibres. Le tout est ensuite refroidi en maintenant le contact avec les premier et second substrats, pour resolidifier l'enrobage externe. Ceci fait adhérer les fibres aux premier et second substrats et on forme ainsi une chambre pour gel entre les premier et second susbtrats. La chambre pour gel a une hauteur définie par le noyau interne des fibres. Les fibres peuvent être chauffées avant ou après avoir placé le second substrat sur les fibres. Les supports pour gel formés peuvent être remplis immédiatement avec une solution formant un gel tel qu'un polyacrylamide, ou ils peuvent être conservés aussi longtemps que nécessaire, en attente d'utilisation.

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