Electrophoresis in low conductivity buffers

Details Electrophoresis in low conductivity buffers Electrophoresis in low conductivity buffers

G - Physics

01

N

G01N 27/447 (2006.01) C12Q 1/68 (2006.01) G01N 27/26 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2162336

Electrophoresis is performed in buffers which exhibit both substantial bufferingcapacity and low electrical conductivity, permitting the separations to be performed at high field strengths without loss of resolution. Four classes of buffering agents are cited as examples: (1) buffering agents with a small number of charged groups per molecule; (2) carrier ampholytes fractionated to a narrow isoelectric point range; (3) low molecular weight buffering ampholytes with an isoelectric point which is close in value to one of the pK values of the ampholyte; and (4) high molecular weight buffering ampholytes in which the acidic and basic groups have the same or very close pK values. These buffering agents are of particular interest in capillary electrophoresis.

Électrophorèse dans des tampons possédant à la fois une grande capacité tampon et une faible conductivité électrique, permettant d'effectuer les séparations à de fortes intensités de champ, sans perte de résolution. On donne comme exemples quatre classes d'agents tampons : 1) agents tampons avec un petit nombre de groupes chargés par molécule; 2) ampholytes véhiculaires, fractionnés selon une plage isoélectrique étroite; 3) ampholytes tampons de faible masse molaire, à point isoélectrique voisin de l'une des valeurs pK de l'ampholyte; 4) ampholytes tampons, de masse molaire élevée, dont les groupes acides et basiques ont des valeurs pK identiques ou très voisines. Ces agents tampons sont d'un grand intérêt pour l'électrophorèse capillaire.

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