Electrophoresis method and apparatus for separating...

Details Electrophoresis method and apparatus for separating... Electrophoresis method and apparatus for separating...

B - Operations, Transporting

01

D

B01D 57/02 (2006.01)

Patent

CA 2463176

A slab gel electrophoresis apparatus (12) comprises a pair of spaced, confronting plates (14, 16) defining a multilane separation zone (22) adapted to hold a separation medium or gel (23), and an upper loading zone. At least one of the plates is shaped to provide an expanded loading zone (16a-d). The plate-to-date distance within the expanded loading zone is greater than that in the separation zone. A comb (24) is provided with each tooth (28) having a gradual taper beginning at a support member (32) and extending along most of the tooth's length. A sharp taper begins beyond the gradual taper and extends to the end of the tooth. The comb is inserted into the loading zone so that the teeth penetrate an upper portion of the gel, thereby forming a series of fluid-tight, elongate sample-loading apertures of subtantially rectangular cross section having a plate-to-plate width greater than that of the separation zone. This allows 96 lane operation even for ultrathin gels.

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