Electrophoresis methods

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2448728

A separation carrier comprising .beta.-1,3-glucan and/or methyl cellulose; a running buffer comprising the separation carrier; a method of capillary electrophoresis or microchip electrophoresis, wherein a sample comprising macromolecular compounds is run in the presence of the running buffer; a method of capillary electrophoresis or microchip electrophoresis, comprising the step of injecting the sample by an electrical injection or application of pressure; and a method for analyzing macromolecular compounds by the method of capillary electrophoresis or microchip electrophoresis. According to the present invention, it is possible to achieve high resolution quickly. Therefore, the methods are useful in the High Through-put screening analysis of proteins or sugar chains in gene analysis, proteome analysis or glycome analysis, and applicable in medical diagnostic apparatuses and the elucidation of biological functions, mechanisms of onset of diseases, etc.

L'invention concerne des supports de séparation contenant beta -1,3-glucane et/ou méthylcellulose; des tampons électrophorétiques les contenant; un procédé d'électrophorèse capillaire ou microchip, aux termes duquel un échantillon contenant un composé polymère est soumis à l'électrophorèse en présence dudit tampon; un échantillon est introduit par injection électrique ou par pressurisation; un procédé d'analyse d'un composé polymère suivant le procédé d'électrophorèse précité. Ces procédés, qui permettent d'établir rapidement une séparabilité élevée, peuvent être utilisés dans des analyses de criblage à haut rendement de protéines ou de chaînes de sucres en analyse génique, en analyse du protéome ou en analyse du glycome, et appliqués à des appareils d'examens cliniques et à la clarification de fonctions biologiques, de mécanismes d'apparition de maladies, etc.

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