Electrophoresis slab gel enclosure for gradient gels

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01) C07K 1/26 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2148539

Gradient gels are formed in a slab gel enclosure by introducing gel solution through a channel in one of the two or more spacers which set the thickness of the gel. The channel opens into the gel space through an aperture in the inner longitudinal edge of the spacer at approximately mid-length along the edge, and filling of the gel space with the solution is performed with the enclosure rotated onto its side. In certain embodiments of the invention, the opposing spacer contains an aperture as well, plus a channel leading from the aperture to the exterior of the enclosure, for venting of air from the gel space as the space is being filled with the gel-forming liquid.

Gels à gradients formés, dans un boîtier pour plaques de gel, par l'introduction de solution de gel dans un passage pratiqué dans une des deux pièces d'espacement, ou plus, qui servent à régler l'épaisseur du gel. Le passage donne sur l'espace à gel par l'entremise d'une ouverture pratiquée approximativement au milieu du côté longitudinal interne de la pièce d'espacement. L'espace à gel est rempli lorsque le boîtier est placé sur son côté. Dans certaines versions, la pièce d'espacement opposée contient également une ouverture, ainsi qu'un passage menant de l'ouverture à l'extérieur du boîtier, ce qui permet d'évacuer l'air de l'espace pour gel au fur et à mesure qu'il se remplit du liquide destiné à former le gel.

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