Electrophoretic in situ tissue staining

G - Physics – 01 – N

Patent

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G01N 1/30 (2006.01)

Patent

CA 2526126

The present invention introduces a radically different way of accelerating biomolecule conjugates into tissue, and hence towards their targets for purposes of tissue staining. The invention provides for an order of magnitude improvement over the prior art diffusion process used to stain tissue. The invention comprises a method of tissue staining by applying an electric field to a tissue sample in the presence of an electrolyte and biomolecular conjugates of interest suspended in the electrolyte. Typical staining times are reduced to seconds as opposed to 30-120 minutes common in the prior art. The invention is also directed to devices for performing the method.

L'invention concerne une manière radicalement différente d'accélérer les conjugués bioloméculaires dans un tissu, et donc en direction de leurs cibles, et ce, à des fins de coloration d'un tissu. Le procédé de l'invention permet d'obtenir une amélioration sensible par rapport au procédé de diffusion de la technique antérieure, utilisée pour colorer un tissu. L'invention porte sur un procédé de coloration de tissus qui consiste à appliquer un champ électrique sur un échantillon de tissu, en présence d'un électrolytes et de conjugués biomoléculaires d'intérêt suspendus dans l'électrolyte. Les durées de coloration classiques sont réduites à des secondes au lieu des 30-120 minutes courantes de la technique antérieure. L'invention porte également sur des dispositifs pour la mise en oeuvre du procédé.

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