C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C07H 1/08 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2279131
The present invention relates to methods for separating nucleic acids from other cellular debris, especially substances that carry a net positive charge at low pH, by electrophoresis under acid conditions. In the purification method of the present invention, nucleic acids are separated from proteins found in the same biological sample by applying the sample to an electrophesis gel and subjecting the sample to electrophoresis under acid conditions to separate the nucleic acids from the proteins. The optimum pH may differ for different sample types but can be readily determined by those skilled in the art. Preferably, the separation is performed at a pH of about 2 to about 4. More preferably, electrophoresis is carried out at a pH of 2.5
La présente invention concerne des méthodes permettant de séparer les acides nucléiques des débris cellulaires, en particulier les substances portant une charge nette positive à faible pH, par électrophorèse en milieu acide. Dans la méthode de purification de la présente invention, pour séparer les acides nucléiques des protéines dans un même échantillon biologique, on soumet l'échantillon à une électrophorèse sur gel en conditions acides. Le pH optimal peut différer selon le type d'échantillon, mais peut être facilement déterminé par les personnes versées dans l'art. De préférence, la séparation est réalisée à un pH d'environ 2 à 4. Le pH privilégié pour effectuer l'électrophorèse est 2,5.
Backus John Wesley
Kreader Carol Ann
Gowling Lafleur Henderson Llp
Ortho-Clinical Diagnostics Inc.
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