Electropipettor and compensation means for electrophoretic bias

Details Electropipettor and compensation means for electrophoretic bias Electropipettor and compensation means for electrophoretic bias

G - Physics

01

N

G01N 27/453 (2006.01) B01F 13/00 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) B81B 1/00 (2006.01) F04B 17/00 (2006.01) F04B 19/00 (2006.01) G01N 1/00 (2006.01) G01N 27/447 (2006.01) H02N 11/00 (2006.01) B01L 3/02 (2006.01)

Patent

CA 2258481

A channel (140) is divised into portions (142, 144). The sidewalls of each channel portion (142, 144) have surface charges of opposite polarity. The two channel portions (142, 144) are physically connected together by a salt bridge (133), such as a glass frit or gel layer. The salt bridge (133) separates the fluids in channel (140) from an ionic fluid reservoir (135). To impart electroosmotic and electrophoretic forces along the channel (140) between parts A and B, respectively. Additionally, a third electrode (137) is placed in the reservoir (135).

L'invention concerne un conduit (140) divisé en deux parties (142, 144). Les parois latérales de chaque partie (142, 144) ont des charges superficielles de polarité opposée. Ces deux parties (142, 144) sont reliées physiquement par un pont de sel (133) du type fritte de verre ou couche de gel. Le pont de sel (133) sépare les fluides s'écoulant dans le conduit (140) depuis un réservoir à fluide ionique (135). L'objectif est d'exercer des forces électroosmotiques et électrophorétiques le long du conduit (140) entre les zones A et B, respectivement. Une troisième électrode (137) est en outre placée dans le réservoir (135).

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