Electroporation cuvette insert for facilitating...

Details Electroporation cuvette insert for facilitating... Electroporation cuvette insert for facilitating...

C - Chemistry, Metallurgy

12

M

C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 13/00 (2006.01) C12N 15/02 (2006.01)

Patent

CA 2437062

Cuvette inserts adapted and configured to fit within an electroporation cuvette. The inserts each include a support structure that holds a porous membrane. When positioned within the cuvette, the membrane is positioned proximal the cuvette electrodes to facilitate membrane-based fusion of cells. In certain aspects, a tube extends through the support structure to allow for application of negative pressure in a convenient location away from electrode contacts and other components of the cuvette or cuvette holder.

Cette invention concerne des inserts conçus et construits pour se loger dans une cuvette d'électroporation. Ces inserts comprennent chacun une structure support maintenant une membrane poreuse. Dans la cuvette, la membrane est positionnée à proximité des électrodes de cuvette, ce qui facilite la fusion de cellules via la membrane. Dans certains aspects, un tube traversant la structure support permet d'appliquer une pression négative en un point adéquat, à l'écart des contacts d'électrode et autres composants de la cuvette ou de son support.

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