Electroporation cuvette with high field intensity and...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 13/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2670757

An electroporation cuvette is constructed as a cylinder with a filament electrode at the cylinder axis and one or more peripheral electrodes on the internal cylinder wall. An additional cuvette design is one with a plurality of peripheral electrodes distributed at various locations around the circumference of the cuvette wall and no central electrode. The various arrangements of electrodes lead to electric field vectors that are non-uniform and of varying orientation, producing high field intensities and agitation of the cell suspension in the cuvette during electroporation.

L'invention concerne une cuvette d'électroporation construite sous la forme d'un cylindre avec une électrode filament au niveau de l'axe du cylindre et une ou plusieurs électrodes périphériques sur la paroi interne du cylindre. Dans un mode de réalisation, l'invention concerne une conception de cuvette additionnelle avec une pluralité d'électrodes périphériques réparties à divers endroits autour de la périphérie de la paroi de la cuvette sans électrode centrale. Les divers agencements d'électrodes conduisent des vecteurs de champ électrique non uniforme et d'orientation variable, produisant des intensités de champs élevées et une agitation de la suspension de cellules présente dans la cuvette durant l'électroporation.

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