Electroporation method including the use of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/87 (2006.01) C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2483524

An object of the present invention is to establish a transformation method simpler and more rapid than conventional techniques. This object can be solved by a method comprising the steps of a) holding a cell under a pressure different from an atmospheric pressure, and b) placing the cell and a nucleic acid under conditions capable of inducing electroporation. The present invention removes the necessity of culture which is conventionally required after gene transfer. Therefore, the present invention makes it possible to obtain a transformant of species which are impossible or considerably difficult to transform by conventional techniques. The simple transformation method of the present invention easily allows large-scale processing and large- scale analysis for research and development in the art. In addition, the present invention triggers dramatic advances in research, potentially leading to the development of innovative recombinants.

L'invention concerne a pour objet d'offrir un procédé de transformation plus simple et plus rapide que les techniques conventionnelles. La solution proposée consiste en un procédé dont les étapes consistent à a) maintenir une cellule sous une pression différente de la pression atmosphérique, b) placer cette cellule et un acide nucléique dans des conditions permettant d'induire une électroporation. Ce procédé permet de supprimer la mise en culture qui est requise dans les procédés conventionnels après un transfert génique. Il permet par conséquent d'obtenir un transformant d'espèces qui sont impossibles ou très difficiles à transformer avec les techniques conventionnelles. Ce procédé de transformation simple permet un traitement et l'analyse à grande échelle pour la recherche et le développement spécialisés. Ce procédé ouvre en outre la voie à des progrès considérables dans la recherche, en offrant la possibilité de créer des produits de recombinaison innovants.

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