Electrotransfer of nucleic acid into tissue cells

Details Electrotransfer of nucleic acid into tissue cells Electrotransfer of nucleic acid into tissue cells

A - Human Necessities

61

K

A61K 48/00 (2006.01) A61N 1/32 (2006.01)

Patent

CA 2619783

The electrotransfer of a nucleic acid into tissue cells, in particular in a muscle or a tumoral tissue, is carried out by an electric stimulation of the tissue as follows: - first with at least one, preferably a single, pulse of a High Voltage field strength of between 200 and 2000 volts/cm - second with a single pulse of Low Voltage field strength of between 50 and 200 volts/cm and of duration of between 300 ms and 2000 ms.

Cette invention concerne l'électrotransfert d'un acide nucléique dans des cellules tissulaires, en particulier dans un muscle ou des tissus tumoraux, lequel électrotransfert est réalisé par stimulation électrique des tissus de la manière suivante: dans un premier temps avec une, de préférence une seule, impulsion d'une intensité de champ haute tension comprise entre 200 et 2000 volts/cm, et dans un deuxième temps avec une seule impulsion d'intensité de champ basse tension comprise entre 50 et 200 volts/cm pendant 300 à 2000 ms.

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