Element and method for determination of creatinine or creatine

Details Element and method for determination of creatinine or creatine Element and method for determination of creatinine or creatine

G - Physics

01

N

150/15.2, 150/3.

G01N 33/70 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 1311179

-i- ELEMENT AND METHOD FOR DETERMINATION OF CREATININE OR CREATINE Abstract of the Disclosure An analytical element for determining creatinine or creatine is disclosed. The element reduces the interference of lidocaine metabolites. The element comprises an absorbent carrier material containing 200-1,200 I.U./m2 of the enzyme creatinine amidohydrolase, 35,000-65,000 I.U./m2 of the enzyme creatine amidinohydrolase and 2,000-3,500 I.U./m2 of the enzyme sarcosine oxidase and a leuco dye which is capable of providing a detectable dye in the presence of hydrogen peroxide and a peroxidative substance, said creatine amidinohydrolase being present in a manner such that it is substantially inert to said leuco dye; and said creatinine amido- hydrolase being present in a rate limiting amount and all three of said enzymes are present at a pH of about 6.5 to 6.9.

551188

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