C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) B01L 3/00 (2006.01) G01N 33/543 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2083173
2083173 9216659 PCTABS00016 An element has been prepared which is useful for the detection of nucleic acids in various formats. The element has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least 70 ·C and have an average diameter of from 0.1 to 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least 30 ·C less than the glass transition temperature of the first plymer. The adhesive is present in the composition at from 1 to 20 weight percent. This element provides high sensitivity and low background in hybridization and other nucleic acid assays.
Cummins Thomas J.
Donish William H.
Findlay John B.
Hinckley Charles C.
Ponticello Ignazio S.
Cummins Thomas J.
Donish William H.
Eastman Kodak Company
Findlay John B.
Gowling Lafleur Henderson Llp
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