Element and method for the determination of creatinine or...

Details Element and method for the determination of creatinine or... Element and method for the determination of creatinine or...

G - Physics

01

N

150/15.1

G01N 33/52 (2006.01) C12Q 1/34 (2006.01) G01N 33/70 (2006.01)

Patent

CA 1278244

ELEMENT AND METHOD FOR THE DETERMINATION OF CREATININE OR CREATINE Abstract of the Disclosure An analytical element can be used for the determination of either creatinine or creatine or both. The element contains creatinine amidohydro- lase, creatine amidinohydrolase and sarcosine oxi- dase, and a leuco dye which is capable of providing a detectable dye in the presence of hydrogen peroxide and a peroxidative substance. The creatine amidino- hydrolase is present in a manner such that it is sub- stantially inert to the leuco dye. The creatinine amidohydrolase is present in a rate limiting amount. By measuring the amount of dye formation at particu- lar times during the assay, either or both of the analytes can be determined with the same element.

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