Elimination of false negatives in nucleic acid detection

Details Elimination of false negatives in nucleic acid detection Elimination of false negatives in nucleic acid detection

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C12Q 1/28 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2143202

The present invention relates to a method for the elimination of false negative test results in assays for the detection of amplified analyte nucleic acid in a sample. Prior to amplification, an internal control is added to the sample, comprising a nucleic acid distinguishable from the analyte nucleic acid, that can be amplified with the same amplification reagents as the analyte nucleic acid. Preferably the internal control comprises a nucleic acid sequence corresponding to the analyte nucleic acid that has been mutated to discriminate it from the analyte nucleic acid.

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