Elucidation of high affinity, high specificity...

Details Elucidation of high affinity, high specificity... Elucidation of high affinity, high specificity...

C - Chemistry, Metallurgy

07

H

C07H 21/02 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2602602

A method is disclosed to obtain oligonucleotide sequences with high affinity to target molecules. By design, the oligonucleotides have a defined primary and secondary structure. The affinity for binding to target species is classified or quantified by assay measurements using physical measurements rather than being based primarily on separations. Targets include but are not limited to proteins, polymers, biological membranes including cells and organelles and small molecules.

L'invention concerne une méthode pour obtenir des séquences oligonucléotidiques présentant une haute affinité pour des molécules cibles. Par leur conception, les oligonucléotides de l'invention présentent une structure primaire et une structure secondaire définies. L'affinité pour une liaison à des espaces cibles est classifiée ou quantifiée par des mesures d'épreuves biologiques à l'aide de mesures physiques, elles ne sont pas essentiellement basées sur des séparatios. Les cibles de l'invention comprennent, de manière non exhaustive, des protéines, des polymères, des membranes biologiques comprenant des cellules et des organites ainsi que de petites molécules.

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