Embryogenic culture initiation of douglas fir by maltose

Details Embryogenic culture initiation of douglas fir by maltose Embryogenic culture initiation of douglas fir by maltose

A - Human Necessities

01

H

A01H 4/00 (2006.01) A01H 7/00 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2464979

In one aspect, the present invention provides methods for producing Douglas- fir somatic embryos. In one aspect, the methods of the invention comprise the step of culturing Douglas-fir explants in, or on, an initiation medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form embryonal suspensor masses comprising early-stage somatic embryos. In another aspect, the methods of the invention additionally comprise the step of culturing Douglas-fir embryonal suspensor masses in maintenance medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form an established embryonal suspensor mass comprising early-stage somatic embryos.

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