Embryonic and adult cns stem cells

Details Embryonic and adult cns stem cells Embryonic and adult cns stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/08 (2006.01) A61K 35/30 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/94 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2259484

A homologous population of CNS multipotent precursor cells from mammalian embryonic neuroepithelial can be expanded in culture while maintaining their multipotential capacity. Chemically defined conditions can generate higher proportions of neurons, astrocytes or oligodendrocytes. In vitro cultures of region-specific, terminally differentiated, mature neurons derived from stem cell cultures are also described.

Procédé in vitro selon lequel une population homogène de cellules précurseurs pluripotentes du système nerveux central tirées de neuroépithélium embryonnaire mammalien peuvent être multipliées dans une culture tout en conservant leur capacité de pluripotence. Des conditions chimiquement définies permettent de produire des quantités beaucoup plus élevées de neurones, d'astrocytes ou d'oligodendrocytes. La présente invention concerne également des cultures in vitro de neurones matures à terminaison différenciée et spécifiques d'une région, dérivés de cultures de cellules souches.

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