C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/16 (2006.01) A61K 35/12 (2006.01) C12N 5/26 (2006.01) C12N 15/06 (2006.01) C12N 15/08 (2006.01) C12N 15/87 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2262817
An improved method of nuclear transfer involving the transplantation of donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation.
Procédé amélioré de transfert nucléaire consiste à effectuer la transplantation de noyaux de cellules donneuses afin de les introduire dans des ovocytes énucléés d'une espèce différente de la cellule donneuse. Les unités obtenues de transfert nucléaire sont utiles pour produire des cellules souches embryonnaires isogéniques, en particulier, de l'homme. Ces cellules embryonnaires ou de type souche sont utiles pour produire des cellules différenciées souhaitées et pour introduire, supprimer ou modifier des gènes souhaités, par exemple, au niveau d'emplacements spécifiques du génome de ces cellules par recombinaison homologue. Ces cellules, qui peuvent contenir un gène hétérologue, sont particulièrement utiles dans des thérapies de transplantation cellulaire, ainsi que pour la recherche in vitro sur la différenciation cellulaire.
Cibelli Jose
Robl James
Stice Steven L.
Gowling Lafleur Henderson Llp
University Of Massachusetts A. Public Institution Of Higher Educ As Represented By Its Amherst Campus
LandOfFree
Embryonic or stem-like cell lines produced by cross species... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Embryonic or stem-like cell lines produced by cross species..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Embryonic or stem-like cell lines produced by cross species... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1469186