C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12N 5/0735 (2010.01) A61K 35/54 (2006.01) C12N 1/38 (2006.01)
Patent
CA 2277278
The present invention provides a serum-free supplement which supports the growth of embryonic stem cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and isolating embryonic stem cells, methods for producing a transgenic animal, and methods for expressing recombinant protein in embryonic stem cells and transgenic animals.
La présente invention concerne un complément dépourvu de sérum supportant la croissance de cellules souches embryonnaires dans une culture. Elle concerne également un milieu comprenant un milieu basal auquel est ajouté le complément sans sérum de l'invention. En outre, la présente invention a pour objet des procédés permettant de mettre en culture et d'isoler des cellules souches embryonnaires, de produire un animal transgénique, et d'exprimer une protéine de recombinaison dans des cellules souches embryonnaires et des animaux transgéniques.
Goldsborough Mindy D.
Price Paul J.
Tilkins Mary Lynn
Invitrogen Corporation
Life Technologies Inc.
Mbm Intellectual Property Law Llp
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