Embryos thawing by isosmolal mixture

Details Embryos thawing by isosmolal mixture Embryos thawing by isosmolal mixture

A - Human Necessities

01

N

62/1, 167/322, 1

A01N 1/00 (2006.01) A61D 1/00 (2006.01) A61D 19/04 (2006.01) F25D 15/00 (2006.01)

Patent

CA 1168585

Abstract: The present invention relates to a method and apparatus for thawing and transferring frozen embryos to recipients. The method includes the steps of thawing a volume of frozen cryoprotective agent containing the embryo and combining the thawed volume with an effective volumetric amount of diluent solution for diluting the cryoprotective agent. The diluent solution has a concentration of nontoxic nonpermeating diluent which is sufficient to result in a dilution mixture which is substanially isosmolal. The method further includes the step of transferring the dilution mixture and embryo directly into a recipient where the embryo is washed by the dilution mixture. The apparatus includes a container comprising first and second chambers. A volume of a cryoprotective solution having the embryo suspended therein is contained in the first chamber. An effective volumetric amount of diluent solution is contained in the second chamber. A removable separator dividing the container into the first and second chambers is provided and on removal allows the contents of the first and second chambers to combine and form a dilution mixture.

379331

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