C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/01 (2006.01) A61K 39/21 (2006.01) C07K 14/105 (2006.01) C07K 14/16 (2006.01) C07K 14/705 (2006.01) C12N 15/48 (2006.01) C12N 15/86 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2125344
ENCAPSIDATED RECOMBINANT POLIOVIRUS NUCLEIC ACID AND METHODS OF MAKING AND USING SAME Abstract The present invention pertains to a method of encapsidating a recombinant poliovirus nucleic acid to obtain a yield of encapsidated viruses which substantially comprises encapsidated recombinant poliovirus nucleic acid. The method of encapsidating a recombinant poliovirus nucleic acid includes contacting a host cell with a recombinant poliovirus nucleic acid which lacks the nucleotide sequence encoding at least a portion of a protein necessary for encapsidation and an expression vector comprising a nucleic acid which encodes at least a portion of one protein necessary for encapsidation under conditions appropriate for introduction of the recombinant poliovirus nucleic acid and the expression vector into the host cell and obtaining a yield of encapsidated viruses which substantially comprises an encapsidated recombinant poliovirus nucleic acid. A foreign nucleotide sequence is generally substituted for the nucleotide sequence of the poliovirus nucleic acid encoding at least a portion of a protein necessary for encapsidation. The invention further pertains to encapsidated recombinant poliovirus nucleic acids produced by the method of this invention and compositions containing the encapsidated recombinant poliovirus nucleic acid containing a foreign nucleotide sequence for use in a method of stimulating an immune response in a subject to the protein encoded by the foreign nucleotide sequence.
Riches Mckenzie & Herbert Llp
Uab Research Foundation
LandOfFree
Encapsidated recombinant poliovirus nucleic acid and methods... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Encapsidated recombinant poliovirus nucleic acid and methods..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Encapsidated recombinant poliovirus nucleic acid and methods... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1498757