Encapsulation of viable tissue and tissue implantation method

A - Human Necessities – 61 – K

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A61K 9/48 (2006.01) A01N 1/02 (2006.01) A61F 2/02 (2006.01) A61K 9/16 (2006.01) A61K 9/50 (2006.01) A61K 35/12 (2006.01) A61K 35/407 (2006.01) A61K 39/44 (2006.01) A61L 27/20 (2006.01) A61L 27/36 (2006.01) B01J 13/04 (2006.01) B01J 13/08 (2006.01) B01J 13/14 (2006.01) B01J 13/16 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01) C12N 11/04 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1145258

ABSTRACT OF THE DISCLOSURE Viable tissue such as mammalian islets of Langerhans or liver tissue is encapsulated within a semipermeable membrane which allows the passage of oxygen, amino acids and nutrients needed for maintenance and ongoing metabolism of the tissue, but is impermeable to bacteria, lymphocytes, and proteins having a molecular weight above a selected level so as to exclude poten- tially deleterious large molecules such as immunoglobulins. By means of the process, and insulin generating system can be pro- duced wherein mammalian islets are maintained in a viable and protected state within the capsule and excrete hormones. Also, mammalian liver tissue can be encapsulated for use as a body fluid detoxification system. The encapsulated liver tissue is shielded from plasma protein of the type responsible for immuno- logical rejection and from leukocytes and bacteria, but toxins and nutrients required for maintenance and continued normal meta- bolism of the tissue freely traverse the membrane. The capsules may be injected into a suitable site in a mammalian body and act as an artificial organ, e.g., and artificial pancreas. The pro- cess comprises the steps of suspending the material to be encap- sulated in a physiologically compatible medium such as a culture medium containing a water soluble substance which can be gelled into droplets to form discrete, shape-retaining temporary cap- sules, forming a semipermeable membrane about the temporary cap- sules, and optionally, reliquify the gelled interior, The pro- cess can also be used for encapsulating chemically active sub- stances such as activated charcoal particles and labile biolog- ical materials.

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