Endomannosidases in the modification of glycoproteins in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/56 (2006.01) C07K 19/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/63 (2006.01) C12N 15/81 (2006.01) C12P 19/14 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2516527

The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.

L'invention concerne des méthodes de modification des structures de glycosylation de protéines recombinantes exprimées dans des champignons ou dans d'autres eukaryotes inférieurs, destinées à ce que cette glycosylation ressemble plus étroitement à la glycosylation de protéines provenant de mammifères supérieurs, en particulier d'humains. L'invention concerne également de nouvelles enzymes et des acides nucléiques les codant, ainsi que des hôtes modifiés pour exprimer ces enzymes, des méthodes de production de glycoprotéines modifiées dans des hôtes et les glycoprotéines modifiées ainsi produites.

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