Endopeptidase

Details Endopeptidase Endopeptidase

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) C07K 14/46 (2006.01) C12N 9/64 (2006.01) C12P 21/00 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2083702

2083702 9218516 PCTABS00016 There is provided by the invention a novel composition comprising, in at least partially purified form, an endopeptidase endogenous to biological cells. The endopeptidase is characterized by having a molecular weight of about 110,000 daltons as measured by SDS PAGE; by being substantially insensitive to PMSF, TPCK, E-64, leupeptin, bacitracin, phosphoramidon and pepstatin; by being substantially inhibited by EDTA and 1,10-phenanthroline; and by being capable of cleaving peptide substrate comprising an alpha helical structural of at least about twelve to fourteen amino acids wherein said helix has a hydrophobic face and a hydrophilic face, said cleavage occurring amino terminal to a lysine or arginine residue on the hydrophilic face positioned within the context of at least four nonpolar amino acids substantially aligned along the hydrophobic face of the helix.

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