Endothelial cell growth factor, methods of isolation and...

Details Endothelial cell growth factor, methods of isolation and... Endothelial cell growth factor, methods of isolation and...

C - Chemistry, Metallurgy

07

K

C07K 14/475 (2006.01) C12N 5/071 (2010.01) A61K 38/10 (2006.01) A61K 38/18 (2006.01) C07K 1/16 (2006.01) C07K 1/30 (2006.01) C07K 7/08 (2006.01) C12N 15/18 (2006.01)

Patent

CA 2050318

A novel growth factor specific for vascular endothelial cells has been identified in conditioned medium of bovine pituitarly derived folliculo stellate cells. This factor, named folliculo stellate derived growth factor (FSdGF) or vascular endothelial growth factor (VEGF), was purified to homogeneity by a combination of exclusion chromatography. Mono S ion exchange chromatography and hydrophobic chromatography on a C4 reverse phase HPLC column. The factor is also found in the murine AtT-20 cell line. FSdGF, having a molecular weight of about 43,000 daltons, was characterized as a glycoprotein composed of two homologous subunits with a molecular weight of about 23,000 daltons.

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