Endothelin dna and use thereof

Details Endothelin dna and use thereof Endothelin dna and use thereof

C - Chemistry, Metallurgy

12

N

530/15.06, 195/1

C12N 15/18 (2006.01) A61K 38/18 (2006.01) C07K 14/475 (2006.01) C07K 14/575 (2006.01) C12N 1/21 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2001303

Disclosed are (1) a DNA sequence containing a DNA segment coding for endothelia-2 or human endothelia-3, (2) a precursor protein and a mature peptide of endothelia-2 or human endothelia-3, (3) a transformant carrying a DNA sequence containing a DNA segment coding for endothelia-2 or human endothelia-3 and (4) a method for preparing mature endothelia-2 or endothelia-3 which comprises culturing the transformant described in (3), producing and accumulating a protein in a culture medium, and collecting the same. Cells transfected or transformed with the DNA allow large amounts of endothelia-2 or endothelia-3 to be produced, which causes the advantageous production of endothelia-2 or endothelin-3. Endothelia-2 and endothelia-3 can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

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