Endotoxin analysis

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) G01N 33/15 (2006.01)

Patent

CA 2628242

The present invention relates to a method for detecting the presence or non- presence of an endotoxin, characterized in that an OmpT protein is br ought into contact with a sample suspected of containing an endotoxin and the protease activity of the OmpT protein is assayed. It also relates to a method for detecting early onset of septicaemia using the inventive method and a kit for performing the method.

L'invention se rapporte à un procédé permettant de détecter la présence ou l'absence d'une endotoxine, caractérisé en ce qu'il consiste à mettre en contact une protéine OmpT avec un échantillon susceptible de contenir une endotoxine, et à détecter l'activité protéase de la protéine OmpT. L'invention concerne également une méthode permettant la détection précoce du début d'une septicémie à l'aide du procédé décrit, et une trousse permettant la mise en oeuvre de cette méthode.

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