Endotoxin removal

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/14 (2006.01) A61K 38/00 (2006.01) A61K 47/48 (2006.01) B01D 15/12 (2006.01) B01D 36/00 (2006.01) C07K 1/30 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2684328

Methods and solutions for removing endotoxins from samples comprising recombinant proteins To the sample comprising said proteins, a solution of yeast extract and salt buffer is provided and the pH is adjusted such that the proteins remain soluble and the endotoxins are precipiated from the solution.

L'invention porte sur des procédés et des solutions pour éliminer des endotoxines d'échantillons comprenant des protéines recombinantes. Une solution d'extrait de levure et un tampon salin est ajoutée à l'échantillon comprenant lesdites protéines et le pH est ajusté de telle sorte que les protéines restent solubles et que les endotoxines sont précipitées de la solution.

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