Energy transfer dyes with enhanced fluorescence

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C09B 11/08 (2006.01) C09B 11/24 (2006.01) C09B 23/00 (2006.01) C09B 47/04 (2006.01) C09B 57/00 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2297589

Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z,C(O)R22R28 where R2, is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z, is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.

On propose de nouveaux coupleurs permettant de lier un colorant donneur à un accepteur dans un colorant fluorescent à transfert d'énergie. Ces coupleurs facilitent le transfert efficace d'énergie entre un colorant donneur et un colorant accepteur dans un colorant à transfert d'énergie. L'un de ces coupleurs permettant de lier un colorant donneur à un colorant accepteur dans un colorant fluorescent à transfert d'énergie possède la structure générale R21Z,C(O)R22R28, où R2 est un alkyle en C1 à C5 lié au colorant donneur, C(O) est un groupement carbonyle, Z est un NH, un soufre ou un oxygène, R22 est un substituant comprenant un alcène, un diène, un alcyne et un cycle à 5 ou 6 atomes possédant au moins un lien insaturé ou une structure cyclique condensée lié au groupement carbonyle, et R28 comprend un groupement fonctionnel reliant le coupleur au colorant accepteur.

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