Engineered antigen-presenting cells expressing an array of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) C12N 5/078 (2010.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2340085

The present invention provides a method for presentation of multiple disease associated antigens in antigen-presenting cells which can be used to generate a prophylactic or therapeutic immune response against the disease with which the antigens are associated. The method employs differential screening of nucleic acid sequences expressed by target and non-target cells. By identifying nucleic acid sequences preferentially expressed in a target cell population and expressing the identified sequences in antigen-presenting cells, one can stimulate an immune response directed at a target cell population without being limited to single, previously identified antigens.

L'invention concerne une méthode de présentation d'antigènes associés à des maladies multiples, présents dans des cellules présentatrices d'antigènes, méthode pouvant être utilisée pour la génération d'une réponse prophylactique ou thérapeutique contre la maladie à laquelle les antigènes sont associés. Ladite méthode consiste à utiliser le criblage différentiel de séquences nucléotidiques exprimées par des cellules cibles et non-cibles. Par l'identification de séquences nucléotidiques exprimées de préférence dans une population de cellules cibles et par l'expression des séquences identifiées, dans les cellules présentatrices d'antigènes, on peut stimuler une réponse immunitaire dirigée contre une population de cellules, sans être limité à certains antigènes particuliers préalablement identifiés.

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