C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) A61K 47/48 (2006.01) C07K 1/14 (2006.01) C07K 19/00 (2006.01) C12N 15/87 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2534629
The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.
L'invention concerne l'identification d'un prodomaine de protéase capable de liaison avec une protéase correspondante avec une affinité élevée. Ce prodomaine est fusionné avec une seconde protéine pour former une protéine de fusion de prodomaine de protéase. La présence d'une protéine de prodomaine de protéase dans une protéine de fusion permet la purification facile et sélective de la seconde protéine par incubation avec la protéase correspondante.
Kirby Eades Gale Baker
University Of Maryland - Biotechnology Institute
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