Engineered zinc finger proteins targeting 5-enolpyruvyl...

Details Engineered zinc finger proteins targeting 5-enolpyruvyl... Engineered zinc finger proteins targeting 5-enolpyruvyl...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/22 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2699769

The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

La présente invention porte sur des protéines à doigt de zinc synthétisées qui ciblent des gènes de 5-énolpyruvyl shikimate-3-phosphate synthase (EPSPS) dans des plantes et sur des procédés d'utilisation de telles protéines à doigt de zinc dans la modulation de l'expression génique, de l'inactivation génique et de la modification de gène ciblées. En particulier, l'invention porte sur des nucléases à doigt de zinc pour un clivage ciblé et une altération des gènes EPSPS.

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