C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2339517
Methods of altering substrate specificity of beta-ketoacyl-ACP synthase, and engineered beta-ketoacyl-ACP synthases so produced are provided. DNA sequences and constructs for expression of engineered beta-ketoacyl-ACP synthases, as well as the novel beta-ketoacyl-ACP synthases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered beta- ketoacyl-ACP synthases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition.
L'invention porte sur des procédés de modification de la spécificité du substrat de la synthase bêta-cétoacyl-ACP, et sur des synthases de bêta-cétoacyl-ACP mises au point. L'invention porte également sur des séquences d'ADN et sur des produits de recombinaison dans l'expression des synthases bêta-cétoacyl-ACP mises au point, ainsi que sur de nouvelles synthases bêta-cétoaxyl-ACP obtenues à partir de ces procédés. Ces séquences d'ADN peuvent être utilisées dans l'expression des synthases bêta-cétoacyl-ACP mises au point des cellules hôtes, notamment des cellules de graines de plantes de récoltes oléagineuses, en vue de modifier la composition des acides gras.
Dehesh Katayoon
Val Dale
Calgene Llc
Mcfadden Fincham
LandOfFree
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